!Name=Sunshine et al 2016 !ExptSetNo=7205 !Description=CGH arrays for Aneuploidy shortens replicative lifespan in Saccharomyces cerevisiae, Sunshine et al Aging Cell 2016. Aneuploidy and aging are correlated; however, a causal link between these two phenomena has remained elusive. Here, we show that yeast disomic for a single native yeast chromosome generally have a decreased replicative lifespan. In addition, the extent of this lifespan deficit correlates with the size of the extra chromosome. We identified a mutation in BUL1 that rescues both the lifespan deficit and a protein trafficking defect in yeast disomic for chromosome 5. Bul1 is an E4 ubiquitin ligase adaptor involved in a protein quality control pathway that targets membrane proteins for endocytosis and destruction in the lysosomal vacuole, thereby maintaining protein homeostasis. Concurrent suppression of the aging and trafficking phenotypes suggests that disrupted membrane protein homeostasis in aneuploid yeast may contribute to their accelerated aging. The data reported here demonstrate that aneuploidy can impair protein homeostasis, shorten lifespan, and may contribute to age-associated phenotypes. !Name=chr 5 disome !Exptid=121243 !Name=chr 5 disome evolved clone 2 !Exptid=121242 !Name=YMD285 x YMD2566 zygote 2 segregant 10A !Exptid=121642 !Name=YMD285 x YMD2566 zygote 1 segregant 7C !Exptid=121670 !Name=YMD285 x YMD2566 zygote 3 segregant 1C !Exptid=121649 !Name=YMD285 x YMD2566 zygote 1 segregant 3B !Exptid=121641 !Name=YMD285 x YMD2566 zygote 1 segregant 4A !Exptid=121643 !Name=YMD285 x YMD2566 zygote 2 segregant 7A !Exptid=121672 !Name=YMD285 x YMD2566 zygote 3 segregant 9C !Exptid=121650 !Name=YMD285 x YMD2566 zygote 3 segregant 5C !Exptid=121652 !Name=YMD285 x YMD2566 zygote 2 segregant 3A !Exptid=121644 !Name=YMD285 x YMD2566 zygote 1 segregant 5D !Exptid=121669 !Name=YMD285 x YMD2566 zygote 1 segregant 8D !Exptid=121671 !Name=YMD285 x YMD2566 zygote 3 segregant 10A !Exptid=121651 !Name=w303 + BUL1Δ c1 !Exptid=121792 !Name=w303 + BUL1Δ c2 !Exptid=121793 !Name=chr 5 disome + BUL1Δ c1 !Exptid=121786 !Name=chr 5 disome + BUL1Δ c2 !Exptid=121787 !Name=chr 8 disome + BUL1Δ c1 !Exptid=121788 !Name=chr 9 disome + BUL1Δ c1 !Exptid=121789 !Name=chr 11 disome + BUL1Δ c1 !Exptid=121791 !Name=YMD671+BUL1 Q146K c1 !Exptid=122042 !Name=w303 + BUL1 Q146K c2 !Exptid=122043 !Name=YMD692+BUL1 Q146K c2 !Exptid=121825