!Citation=McIsaac RS, et al. (2012) Mol. Biol. Cell June 13, 2012 mbc.E12-03-0232 !Title=Perturbation-based analysis and modeling of combinatorial regulation in the yeast sulfur assimilation pathway. !PubMedID=22696683 !Name=Induction_MethionineData !ExptSetNo=6884 !Description=RNA extraction, labeling, and hybridization were performed as in Brauer et al. with slight modifications (Brauer et al., 2008). Samples from chemostat cultures (5 mL) were vacuum-filtered onto 0.45-μm nylon membranes (Millipore, HNWP02500), placed in 2mL locking lid tubes 20 (FisherBrand, 02-681-291), and then flash-frozen in liquid nitrogen. Samples were stored at -80?C until RNA extraction, which was performed with a standard acid-phenol procedure. Crude RNA was cleaned with RNeasy mini columns (QIAGEN, Valencia, CA) prior to mRNA amplification and labeling with the Agilent Quick-Amp Labeling Kit (Part No. 5190-0424). Amplification and labeling were performed per manufacturer?s instructions but with half the volume of each reagent and 0.6 μL of Cy3 or Cy5 dye. Reference RNA was extracted from a lab WT strain (DBY12001) grown to steady state in phosphate-limited (20 mg/L) growth medium at D = 0.18 h-1. Agilent Yeast Oligo V2 microarrays (8x15k) were hybridized for 17 hours at 65?C on a rotisserie at 20RPM. Hybridized microarrays were washed, scanned, and raw data were extracted with Agilent Feature Extractor Software version 9.5. This methionine-limited overexpression dataset consists of 40 microarrays and five separate time courses. For each of 5 TFs (and one GEV-only control, DBY12142) we hybridized mRNA before (t = 0 min) and following (t = 2.5, 5, 15, 30, 45, 60, and 90 min.) addition of 1 μM β-estradiol.