!Name=Induction_MethionineData !ExptSetNo=6884 !Description=RNA extraction, labeling, and hybridization were performed as in Brauer et al. with slight modifications (Brauer et al., 2008). Samples from chemostat cultures (5 mL) were vacuum-filtered onto 0.45-μm nylon membranes (Millipore, HNWP02500), placed in 2mL locking lid tubes 20 (FisherBrand, 02-681-291), and then flash-frozen in liquid nitrogen. Samples were stored at -80?C until RNA extraction, which was performed with a standard acid-phenol procedure. Crude RNA was cleaned with RNeasy mini columns (QIAGEN, Valencia, CA) prior to mRNA amplification and labeling with the Agilent Quick-Amp Labeling Kit (Part No. 5190-0424). Amplification and labeling were performed per manufacturer?s instructions but with half the volume of each reagent and 0.6 μL of Cy3 or Cy5 dye. Reference RNA was extracted from a lab WT strain (DBY12001) grown to steady state in phosphate-limited (20 mg/L) growth medium at D = 0.18 h-1. Agilent Yeast Oligo V2 microarrays (8x15k) were hybridized for 17 hours at 65?C on a rotisserie at 20RPM. Hybridized microarrays were washed, scanned, and raw data were extracted with Agilent Feature Extractor Software version 9.5. This methionine-limited overexpression dataset consists of 40 microarrays and five separate time courses. For each of 5 TFs (and one GEV-only control, DBY12142) we hybridized mRNA before (t = 0 min) and following (t = 2.5, 5, 15, 30, 45, 60, and 90 min.) addition of 1 μM β-estradiol. !Name=Jo_Cbf1_DBY12091_1_1-0 !Exptid=115649 !Name=Jo_Cbf1_DBY12091_1_2-2.5 !Exptid=115650 !Name=Jo_Cbf1_DBY12091_1_3-5 !Exptid=115651 !Name=Jo_Cbf1_DBY12091_1_4-15 !Exptid=115652 !Name=Jo_Cbf1_DBY12091_2_1-30 !Exptid=115653 !Name=Jo_Cbf1_DBY12091_2_2-45 !Exptid=115654 !Name=Jo_Cbf1_DBY12091_2_3-60 !Exptid=115655 !Name=Jo_Cbf1_DBY12091_2_4-90 !Exptid=115656 !Name=Met31_DBY12092_1_1-0_MetLimited !Exptid=115710 !Name=Met31_DBY12092_1_2-2.5 !Exptid=115711 !Name=Met31_DBY12092_1_3-5 !Exptid=115712 !Name=Met31_DBY12092_1_4-15 !Exptid=115713 !Name=Met31_DBY12092_2_1-30 !Exptid=115714 !Name=Met31_DBY12092_2_2-45 !Exptid=115715 !Name=Met31_DBY12092_2_3-60 !Exptid=115716 !Name=Met31_DBY12092_2_4-90 !Exptid=115717 !Name=Met32_DBY12093_1_1-0_MetLimited !Exptid=115727 !Name=Met32_DBY12093_1_2-2.5_MetLimited !Exptid=115728 !Name=Met32_DBY12093_1_3-5_MetLimited !Exptid=115729 !Name=Met32_DBY12093_1_4-15_MetLimited !Exptid=115730 !Name=Met32_DBY12093_2_1-30_MetLimited !Exptid=115734 !Name=Met32_DBY12093_2_2-45_MetLimited !Exptid=115736 !Name=Met32_DBY12093_2_3-60_MetLimited !Exptid=115738 !Name=Met32_DBY12093_2_4-90_MetLimited !Exptid=115732 !Name=Met28_DBY12094_1_1-0_MetLimited !Exptid=115731 !Name=Met28_DBY12094_1_2-2.5_MetLimited !Exptid=115733 !Name=Met28_DBY12094_1_3-5_MetLimited !Exptid=115735 !Name=Met28_DBY12094_1_4-15_MetLimited !Exptid=115737 !Name=Met28_DBY12094_2_1-30_MetLimited !Exptid=115739 !Name=Met28_DBY12094_2_2-45_MetLimited !Exptid=115740 !Name=Met28_DBY12094_2_3-60_MetLimited !Exptid=115741 !Name=Met28_DBY12094_2_4-90_MetLimited !Exptid=115742 !Name=Jo_Met4_DBY12099_1_1-0 !Exptid=115610 !Name=Jo_Met4_DBY12099_1_2-2.5 !Exptid=115611 !Name=Jo_Met4_DBY12099_1_3-5 !Exptid=115612 !Name=Jo_Met4_DBY12099_1_4-15 !Exptid=115613 !Name=Jo_Met4_DBY12099_2_1-30 !Exptid=115614 !Name=Jo_Met4_DBY12099_2_2-45 !Exptid=115615 !Name=Jo_Met4_DBY12099_2_3-60 !Exptid=115616 !Name=Jo_Met4_DBY12099_2_4-90 !Exptid=115617 !Name=WT_DBY12142_1_1-0_MetLimited !Exptid=115718 !Name=WT_DBY12142_1_2-2.5_MetLimited !Exptid=115719 !Name=WT_DBY12142_1_3-5_MetLimited !Exptid=115720 !Name=WT_DBY12142_1_4-15_MetLimited !Exptid=115721 !Name=WT_DBY12142_2_1-30_MetLimited !Exptid=115722 !Name=WT_DBY12142_2_2-45_MetLimited !Exptid=115723 !Name=WT_DBY12142_2_3-60_MetLimited !Exptid=115724 !Name=WT_DBY12142_2_4-90_MetLimited !Exptid=115725 !Name=WT_DBY12142_1_1-120_MetLimited !Exptid=115726