!Citation=Oliveira RP, et al. (2009) Aging Cell Jul 3. !Title=Condition-adapted stress and longevity gene regulation by Caenorhabditis elegans SKN-1/Nrf. !PubMedID=19575768 !Name=Oliveira et. al. 2009 !ExptSetNo=6137 !Description=For each microarray experiment, total RNA was isolated from 50 000 animals using Trizol (Invitrogen, Carlsbard, CA, USA). cDNA was synthesized and linearly amplified from 325 ng RNA using the Low RNA Input Linear Amplification Kit (Agilent, Santa Clara, CA, USA), and labeled with Cy3- or Cy5-CTP (Perkin Elmer, Waltham, MA, USA). A dye swap analysis was performed for each set of biological replicate samples. Samples were fragmented according to Agilent protocols and hybridized overnight at 60 ?C to Agilent-015061: C. elegans oligonucleotide Microarray 4 ? 44 arrays (covering 21 481 genes). Array scanning was performed using a DNA Microarray Scanner (Agilent) at 5 μm resolution. The output image was processed by Feature Extractor (Agilent) and normalized for dye bias by linear correction using rank consistent probes. Prior to hierarchical clustering, values from spots on the microarray that represented the same gene were averaged to a single value. Spots flagged by the Feature Extractor software as having red and green intensities well above background were omitted. Finally, genes that did not have an observed absolute value of 0.4 for the log(base2) ratio of red/green intensities for at least one array were omitted, as were any genes that lacked information for > 20% of the arrays. After filtering, the remaining genes were submitted for downstream analysis.