!Citation=Capra EJ, et al. (2008) PLoS ONE 3(12):e4055 !Title=Comparative developmental expression profiling of two C. elegans isolates. !PubMedID=19116648 !Name=final n2 and hawaii !ExptSetNo=5601 !Description=RNA was labeled using the Low RNA Input Fluorescent Linear Amplification Kit (Agilent, San Jose CA) according to the manufacturer's instructions. The reference used is a 50:50 N2:CB4856 combination of RNA isolated from separate plates of hermaphrodite only mixed-stage populations. Four replicates of each time point were completed. For each time point two of the experimental samples were labeled with Cy3 while two were labeled with Cy5. 850 ng of an experimental sample and of the reference were hybridized to Agilent C. elegans 4?44k oligo microarrays for each array, according to the manufacturer's protocol. Samples were loaded into each array randomly on the slide, so that each slide did not contain more than one sample from each time point. The slides were scanned using an Agilent DNA microarray scanner and the data was extracted using Agilent Feature Extractor (version 9.5).