!Name=PfAdoMetDC/ODC co-inhibition
!ExptSetNo=5395
!Description=PfAdoMetDC/ODC co-inhibition of 3D7 Plasmodium falciparum cultures was performed with 5mM DFMO and 5uM MDL73811 alongside untreated controls in duplicate (2 biological replicates). Treatment was initiated in the schizont stage [42 hours post invasion (hpi)] and cultures were sampled based on morphology of the untreated parasites at t1 = 19hpi, t2 = 27hpi and t3 = 34hpi of the next cycle approximately. RNA was extracted and a common reference RNA pool was prepared. cDNA was prepared as described (Bozdech, 2003). Sample cDNA was labeled with Cy5 and the common reference with Cy3. Oligonucleotide hybridization was perfomed at 65 C overnight, followed by washing and scanning with an Axon GenePix 4000A scanner. Technical replication of the hybridizations was performed where possible.
!Name=2006-11-02 3D7 Drug Treatment Control A unamplified time point 19 (hr)
!Exptid=104962
!Name=2006-11-02 3D7 Drug Treatment Control A unamplified (tech repl) time point 19 (hr)
!Exptid=104987
!Name=2006-11-02 3D7 Drug Treatment Control B unamplified time point 19 (hr)
!Exptid=104918
!Name=2006-11-02 3D7 Drug Treatment Control A unamplified time point 27 (hr)
!Exptid=104963
!Name=2006-11-02 3D7 Drug Treatment Control A unamplified (tech repl) time point 27 (hr)
!Exptid=104988
!Name=2006-11-02 3D7 Drug Treatment Control B unamplified time point 27 (hr)
!Exptid=104919
!Name=2006-11-02 3D7 Drug Treatment Control A unamplified time point 34 (hr)
!Exptid=104964
!Name=2006-11-02 3D7 Drug Treatment Control A unamplified (tech repl) time point 34 (hr)
!Exptid=104989
!Name=2006-11-02 3D7 Drug Treatment Control B unamplified time point 34 (hr)
!Exptid=104920
!Name=2006-11-02 3D7 Drug Treatment Control B unamplified (tech repl) time point 34 (hr)
!Exptid=104991
!Name=2006-11-02 3D7 Drug Treatment 5uM MDL73811 + 5mM DFMO B unamplified time point 19 (hr) NEW UPLOAD
!Exptid=105891
!Name=2006-11-02 3D7 Drug Treatment 5uM MDL73811 + 5mM DFMO B unamplified time point 19 (hr)
!Exptid=104921
!Name=2006-11-02 3D7 Drug Treatment 5uM MDL73811 + 5mM DFMO A unamplified time point 27 (hr)
!Exptid=104966
!Name=2006-11-02 3D7 Drug Treatment 5uM MDL73811 + 5mM DFMO A unamplified (tech repl) time point 27 (hr)
!Exptid=104992
!Name=2006-11-02 3D7 Drug Treatment 5uM MDL73811 + 5mM DFMO B unamplified time point 27 (hr)
!Exptid=104922
!Name=2006-11-02 3D7 Drug Treatment 5uM MDL73811 + 5mM DFMO B unamplified (tech repl) time point 27 (hr)
!Exptid=104994
!Name=2006-11-02 3D7 Drug Treatment 5uM MDL73811 + 5mM DFMO A unamplified time point 34 (hr)
!Exptid=104967
!Name=2006-11-02 3D7 Drug Treatment 5uM MDL73811 + 5mM DFMO A unamplified (tech repl) time point 34 (hr)
!Exptid=104993
!Name=2006-11-02 3D7 Drug Treatment 5uM MDL73811 + 5mM DFMO B unamplified time point 34 (hr)
!Exptid=104923
!Name=2006-11-02 3D7 Drug Treatment 5uM MDL73811 + 5mM DFMO B unamplified (tech repl) time point 34 (hr)
!Exptid=104995