!Name=Effect of pseudorabies virus infection and interferon treatment on transcriptional responses !ExptSetNo=5125 !Description=The goal of this experiment was to determine the effect of PRV Becker infection on the expression of cellular IFN-stimulated genes. Growth-arrested rat embryonic fibroblasts were either (1) mock-treated, (2) infected with PRV Becker at 5 pfu/cell, (3) treated with 1000 U/ml rat IFN beta, or (4) simultaneously infected with PRV Becker and treated with IFN. Two timepoints were tested: 3 and 12 hrs post treatment. At 12 hrs, a large subset of genes that were induced 3-fold or more by IFN relative to their levels in mock-treated cells was not induced when the cells were simultaneously infected with PRV. Expression of transcripts associated with MHC class I antigen presentation and NK cell activation was reduced while transcipts associated with inflammation were unaffected or were induced by viral infection. !Name=mock-3H-A !Exptid=103522 !Name=mock-3H-B !Exptid=103523 !Name=mock-3H-C !Exptid=103524 !Name=mock-3H-D !Exptid=103525 !Name=Be-3H-A !Exptid=103526 !Name=Be-3H-B !Exptid=103527 !Name=Be-3H-C !Exptid=103528 !Name=mock-12H-A !Exptid=103529 !Name=mock-12H-B !Exptid=103530 !Name=mock-12H-C !Exptid=103531 !Name=Be-12H-A !Exptid=103532 !Name=Be-12H-B !Exptid=103533 !Name=Be-12H-C !Exptid=103534 !Name=IFN-12H-A !Exptid=103535 !Name=IFN-12H-B !Exptid=103536 !Name=IFN-12H-C !Exptid=103537 !Name=Be-IFN-12H-A !Exptid=103538 !Name=Be-IFN-12H-B !Exptid=103539