!Name=Gasch et al.(2000):Yeast Stress Response !ExptSetNo=148 !Description=We explored genomic expression patterns in the yeast Saccharomyces cerevisiae responding to diverse environmental transitions. DNA microarrays were used to measure changes in transcript levels over time for almost every yeast gene, as cells responded to temperature shocks, hydrogen peroxide, the superoxide-generating drug menadione, the sulfhydryl-oxidizing agent diamide, the disulfide-reducing agent dithiothreitol, hyper- and hypo-osmotic shock, amino acid starvation, nitrogen source depletion, and progression into stationary phase. A large set of genes ( approximately 900) showed a similar drastic response to almost all of these environmental changes. Additional features of the genomic responses were specialized for specific conditions. Promoter analysis and subsequentcharacterization of the responses of mutant strains implicated the transcription factors Yap1p, as well as Msn2p and Msn4p, in mediating specific features of the transcriptional response, while the identification of novel sequence elements provided clues to novel regulators. Physiological themes in the genomic responses to specific environmental stresses provided insights into the effects of those stresses on the cell. !Name=heat shock 05 min !Exptid=106 !Name=heat shock 10 min !Exptid=107 !Name=heat shock 15 min !Exptid=108 !Name=heat shock 20 min repeat !Exptid=115 !Name=heat shock 20 min !Exptid=109 !Name=heat shock 40 min !Exptid=116 !Name=heat shock 60 min !Exptid=117 !Name=heat shock 80 min !Exptid=118 !Name=Heat Shock 000 minutes (series 2) !Exptid=781 !Name=Heat Shock 000 minutes !Exptid=497 !Name=Heat Shock 000 minutes (series 3) !Exptid=797 !Name=Heat Shock 005 minutes !Exptid=799 !Name=Heat Shock 015 minutes !Exptid=800 !Name=Heat Shock 030inutes !Exptid=801 !Name=Heat Shock 060 minutes !Exptid=802 !Name=25C -- 15 min !Exptid=7535 !Name=25C -- 30 min !Exptid=7536 !Name=25C -- 45 min !Exptid=7537 !Name=25C -- 60 min !Exptid=7538 !Name=25C -- 90 min !Exptid=7539 !Name=29C to 33C - 5 min !Exptid=2559 !Name=29C to 33C - 15 min !Exptid=2560 !Name=29C to 33C -30 min !Exptid=4783 !Name=29C to 33C - 90 min !Exptid=4784 !Name=29C +1M sorbitol to 33C + 1M sorbitol - 15 min !Exptid=4785 !Name=29C +1M sorbitol to 33C + 1M sorbitol - 5 min !Exptid=4786 !Name=29C +1M sorbitol to 33C + 1M sorbitol - 30 min !Exptid=4787 !Name=33C no sorbitol 5 min !Exptid=7549 !Name=33C + 1M sorbitol vs. 29C - adapted !Exptid=4789 !Name=33C no sorbitol 30 min !Exptid=7547 !Name=heat shock 17 to 37, 20 minutes !Exptid=12744 !Name=heat shock 21 to 37, 20 minutes !Exptid=410 !Name=heat shock 25 to 37, 20 minutes !Exptid=409 !Name=heat shock 29 to 37, 20 minutes !Exptid=408 !Name=heat shock 33 to 37, 20 minutes !Exptid=407 !Name=heat shock 37 to 37, 20 minutes !Exptid=406 !Name=heat shock 37 to 37, 30 minutes !Exptid=2144 !Name=constant 0.32 mM H2O2 (10 min) redo !Exptid=989 !Name=constant 0.32 mM H2O2 (20 min) redo !Exptid=985 !Name=constant 0.32 mM H2O2 (30 min) redo !Exptid=986 !Name=constant 0.32 mM H2O2 (40 min) rescan !Exptid=6377 !Name=constant 0.32 mM H2O2 (50 min) redo !Exptid=987 !Name=constant 0.32 mM H2O2 (60 min) redo !Exptid=988 !Name=constant 0.32 mM H2O2 (80 min) redo !Exptid=993 !Name=constant 0.32 mM H2O2 (100 min) redo !Exptid=990 !Name=constant 0.32 mM H2O2 (120 min) redo !Exptid=991 !Name=constant 0.32 mM H2O2 (160 min) redo !Exptid=992 !Name=1 mM Menadione (10 min)redo !Exptid=972 !Name=1 mM Menadione (20 min) redo !Exptid=961 !Name=1 mM Menadione (30 min) redo !Exptid=962 !Name=1mM Menadione (40 min) redo !Exptid=971 !Name=1 mM Menadione (50 min)redo !Exptid=1661 !Name=1 mM Menadione (80 min) redo !Exptid=974 !Name=1 mM Menadione (105 min) redo !Exptid=975 !Name=1 mM Menadione (120 min)redo !Exptid=963 !Name=1 mM Menadione (160 min) redo !Exptid=964 !Name=2.5mM DTT 005 min !Exptid=512 !Name=2.5mM DTT 015 min !Exptid=513 !Name=2.5mM DTT 030 min !Exptid=514 !Name=2.5mM DTT 045 min !Exptid=515 !Name=2.5mM DTT 060 min !Exptid=516 !Name=2.5mM DTT 090 min !Exptid=517 !Name=2.5mM DTT 120 min !Exptid=518 !Name=2.5mM DTT 180 min !Exptid=519 !Name=dtt 000 min !Exptid=8227 !Name=dtt 015 min !Exptid=8228 !Name=dtt 030 min !Exptid=8229 !Name=dtt 060 min !Exptid=8230 !Name=dtt 120 min !Exptid=8233 !Name=dtt 240 min !Exptid=8234 !Name=dtt 480 min !Exptid=8235 !Name=1.5 mM diamide (5 min) !Exptid=5357 !Name=1.5 mM diamide (10 min) !Exptid=830 !Name=1.5 mM diamide (20 min) !Exptid=831 !Name=1.5 mM diamide (30 min) !Exptid=834 !Name=1.5 mM diamide (40 min) !Exptid=836 !Name=1.5 mM diamide (50 min) !Exptid=838 !Name=1.5 mM diamide (60 min) !Exptid=840 !Name=1.5 mM diamide (90 min) !Exptid=842 !Name=1M sorbitol - 5 min !Exptid=5358 !Name=1M sorbitol - 15 min !Exptid=815 !Name=1M sorbitol - 30 min !Exptid=817 !Name=1M sorbitol - 45 min (* centrifugation problem) !Exptid=812 !Name=1M sorbitol - 60 min !Exptid=4872 !Name=1M sorbitol - 90 min !Exptid=4874 !Name=Hypo-osmotic shock - 5 min !Exptid=2064 !Name=Hypo-osmotic shock -15 minutes !Exptid=2554 !Name=Hypo-osmotic shock -30 minutes !Exptid=2555 !Name=Hypo-osmotic shock - 45 minutes !Exptid=2556 !Name=Hypo-osmotic shock - 60 minutes !Exptid=2557 !Name=1M sorbitol vs. YPD - basal !Exptid=2558 !Name=Amino acid + adenine starvation 0.5 h !Exptid=862 !Name=Amino acid + adenine starvation 1 h !Exptid=863 !Name=Amino acid + adenine starvation 2 h !Exptid=896 !Name=Amino acid + adenine starvation 4 h !Exptid=864 !Name=Amino acid + adenine starvation 6 h !Exptid=865 !Name=Nitrogen Depletion 30 min. !Exptid=12739 !Name=Nitrogen Depletion 1 h !Exptid=12741 !Name=Nitrogen Depletion 2 h !Exptid=12742 !Name=Nitrogen Depletion 4 h !Exptid=12732 !Name=Nitrogen Depletion 8 h !Exptid=12733 !Name=Nitrogen Depletion 12 h !Exptid=12734 !Name=Nitrogen Depletion 1 d !Exptid=12736 !Name=Nitrogen Depletion 2 d !Exptid=12737 !Name=Nitrogen Depletion 3 d !Exptid=12738 !Name=YPD 2 h (25C) !Exptid=1009 !Name=YPD 4 h (25C) !Exptid=1010 !Name=YPD 8 h (25C) !Exptid=1011 !Name=YPD 12 h (25C) !Exptid=1012 !Name=YPD 1 d (25C) !Exptid=1013 !Name=YPD 2 d (25C) !Exptid=1014 !Name=YPD 3 d (25C) !Exptid=1015 !Name=YPD 5 d (25C) !Exptid=1016 !Name=YPD 7 d (25C) !Exptid=1017 !Name=YPD 13 d (25C) !Exptid=1018 !Name=YPD_4_h_30C !Exptid=853 !Name=YPD_6_h_30C !Exptid=854 !Name=YPD_8_h_30C !Exptid=855 !Name=YPD_10_h_30C !Exptid=856 !Name=YPD_12_h_30C !Exptid=857 !Name=YPD_1_d_30C !Exptid=858 !Name=YPD_2_d_30C !Exptid=859 !Name=YPD_3_d_30C !Exptid=860 !Name=YPD_5_d_30C !Exptid=861 !Name=YP ethanol vs reference pool !Exptid=686 !Name=YP galactose vs reference pool !Exptid=681 !Name=YP raffinose vs reference pool !Exptid=1686 !Name=YP sucrose vs reference pool !Exptid=685 !Name=YP fructose vs reference pool !Exptid=684 !Name=YP glucose vs reference pool !Exptid=680 !Name=17 deg growth !Exptid=584 !Name=21 deg growth !Exptid=585 !Name=25 deg growth !Exptid=597 !Name=29 deg growth !Exptid=598 !Name=37 deg growth !Exptid=599 !Name=steady state 15 dec C !Exptid=8193 !Name=steady state 17 dec C !Exptid=8217 !Name=steady state 21 dec C !Exptid=8195 !Name=steady state 25 dec C !Exptid=8197 !Name=steady state 29 dec C !Exptid=8204 !Name=steady state 33 dec C !Exptid=8210 !Name=steady state 36 dec C: y14n095 !Exptid=8247 !Name=steady state 36 dec C !Exptid=8213 !Name=DBY7286 37degree heat - 20 min !Exptid=809 !Name=DBYmsn2-4- 37degree heat - 20 min !Exptid=814 !Name=DBY msn2msn4 37C - 20 min !Exptid=7530 !Name=DBYyap1- 37degree heat - 20 min (redo) !Exptid=6361 !Name=DBYyap1 + 37degree heat (repeat) !Exptid=1258 !Name=DBY7286 + 0.3 mM H2O2 (20 min) !Exptid=691 !Name=DBYmsn2msn4 (good strain) + 0.32 mM H2O2 !Exptid=12801 !Name=DBY msn2msn4 0.32 mM H2O2 - 20 min !Exptid=7529 !Name=DBYyap1- + 0.3 mM H2O2 (20 min) !Exptid=692 !Name=DBYyap1 0.32 mM H2O2 - 20 min !Exptid=7528 !Name=FY3 pRZ-MSN2 - Msn2 overexpression !Exptid=877 !Name=FY3 pRZ-MSN4 - Msn4 overexpression !Exptid=878 !Name=yap59 !Exptid=8908