!Name=Complete data for : Yoshimoto H, et al. (2002) J Biol Chem !ExptSetNo=1427 !Description=In Saccharomyces cerevisiae, the Ca2+/calmodulin-dependent protein phosphatase, calcineurin, is activated by specific environmental conditions, including exposure to Ca2+ and Na+, and induces gene expression by regulating the Crz1p/Tcn1p transcription factor. We used DNA microarrays to perform a comprehensive analysis of calcineurin/Crz1p-dependent gene expression following addition of Ca2+ (200 mM) or Na+ (0.8 M) to yeast. 163 genes exhibited increased expression that was reduced 50% or more by calcineurin inhibition. These calcineurin dependent genes function in signaling pathways, ion/small molecule transport, cell wall maintenance, vesicular transport, and include many open reading frames of heretofore-unknown function. Three distinct gene classes were defined: 28 genes displayed calcineurin-dependent induction in response to Ca2+ and Na+, 125 showed calcineurin-dependent expression following Ca2+ but not Na+ addition, and 10 were regulated by calcineurin in response to Na+ but not Ca2+. Analysis of crz1D cells established Crz1p as the major effecter of calcineurin-regulated gene expression in yeast. We identified the Crz1p binding site as 5-GNGGC(G/T)CA-3 by in vitro site selection. A similar sequence, 5-GAGGCTG-3, was identified as a common sequence motif in the upstream regions of calcineurin/Crz1p-dependent genes. This finding is consistent with direct regulation of these genes by Crz1p. !Name=Ca 5min (1) !Exptid=14711 !Name=Ca 15min (1) !Exptid=14712 !Name=Ca 30min (1) !Exptid=14761 !Name=Ca 60min (1) !Exptid=14763 !Name=Ca 5min (2) !Exptid=15179 !Name=Ca 15min (2) !Exptid=15180 !Name=Ca 30min (2) !Exptid=15181 !Name=Ca 60min (2) !Exptid=15182 !Name=Ca+FK 5min (3) !Exptid=15183 !Name=Ca+FK 15min (5) !Exptid=17472 !Name=Ca+FK 30min (5) !Exptid=17473 !Name=Ca+FK 60min (5) !Exptid=17618 !Name=Ca+FK 5min (2) !Exptid=15184 !Name=Ca+FK 15min (2) !Exptid=15635 !Name=Ca+FK 30min (2) !Exptid=15636 !Name=Ca+FK 60min (2) !Exptid=15638 !Name=Ca/Ca+FK 15min (1) !Exptid=13069 !Name=Ca/Ca+FK 30min (1) !Exptid=13072 !Name=Ca/Ca+FK 15min (2) !Exptid=13070 !Name=Ca/Ca+FK 30min (2) !Exptid=13133 !Name=wt/crz1 Ca 15min (1) !Exptid=13061 !Name=wt/crz1 Ca 30min (1) !Exptid=13063 !Name=wt/crz1 Ca 15min (2) !Exptid=13062 !Name=wt/crz1 Ca 30min (2) !Exptid=13064 !Name=Na 15min !Exptid=7758 !Name=Na 30min !Exptid=7759 !Name=Na 45min !Exptid=7760 !Name=Na 60min !Exptid=7761 !Name=Na+FK 15min !Exptid=7762 !Name=Na+FK 30min !Exptid=7763 !Name=Na+FK 45min !Exptid=7764 !Name=Na+FK 60min !Exptid=7765 !Name=Na/Na+FK 30min (1) !Exptid=13065 !Name=Na/Na+FK 45min (1) !Exptid=13067 !Name=Na/Na+FK 30min (2) !Exptid=13066 !Name=Na/Na+FK 45min (2) !Exptid=13068 !Name=wt/crz1 Na 30min (1) !Exptid=13057 !Name=wt/crz1 Na 45min (1) !Exptid=13059 !Name=wt/crz1 Na 30min (2) !Exptid=13058 !Name=wt/crz1 Na 45min (2) !Exptid=13060