!Citation=Whitfield ML, et al. (2002). Mol Biol Cell 13(6):1977-2000
!Title=Identification of genes periodically expressed in the human cell cycle and their expression in tumors.
!PubMedID=12058064
!Name=Cell Cycle - HeLa S3, Double Thymidine Block, Experiment 1
!ExptSetNo=901
!Description=HeLa S3 cells were synchronized in early S phase with a double thymidine block as described in Whitfield et al(2002)Mol.Biol.Cell. This experiment set contains the complete set of 24K arrays for the first double thymidine block synchronization (Thy-Thy1).
!Name=Cell Cycle - HeLa S3, Double Thymidine Block, Experiment 2
!ExptSetNo=902
!Description=HeLa S3 cells were synchronized in early S phase with a double thymidine block as described in Whitfield et al(2002)Mol.Biol.Cell. This experiment set contains the complete set of 24K arrays for the second double thymidine block synchronization (Thy-Thy2).
!Name=Cell Cycle - HeLa S3, Double Thymidine Block, Experiment 3
!ExptSetNo=903
!Description=HeLa S3 cells were synchronized in early S phase with a double thymidine block as described in Whitfield et al(2002)Mol.Biol.Cell. This experiment set contains the complete set of 43K arrays for the third double thymidine block synchronization (Thy-Thy3).
!Name=Cell Cycle - HeLa S3, Thymidine-Nocodazole Block
!ExptSetNo=904
!Description=HeLa S3 cells were synchronized in mitosis by arresting first in S phase with thymidine followed by arrest in mitosis with Nocodazole as described in Whitfield et al(2002)Mol.Biol.Cell. This experiment set contains the complete set of 43K arrays for the thymidine-nocodazole synchronization (Thy-Noc).
!Name=Cell Cycle - HeLa S3, Mitotic Selection, Experiment 1
!ExptSetNo=905
!Description=HeLa S3 cells in mitosis were selected by mitotic shake-off using an automated cell shaker (Eliassen et al. 2000, MCB)by Beth Alexander and Myra Hurt at Florida State University. This experiment set contains the complete set of 43K arrays for the first mitotic shake-off experiment described in Whitfield et al.(2002), (Shake).
!Name=Cell Cycle - Complete data for Whitfield et al. (2002) MBC
!ExptSetNo=907
!Description=This experiment set contains the complete set of raw data for Whitfield et al. (2002)Mol.Biol.Cell. HeLa S3 cells were synchronized by three different methods (double thymidine block, thymidine-nocodazole block or mitotic shake-off)in five independent time courses. mRNA from the first and second double thymidine block experiments were converted to cDNA by standard methods using an oligo-dT/random hexamer mix and hybridized to 24K arrays; mRNA from the third double thymidine and thymidine-nocodazole block experiments were converted to cDNA using olido-dT alone and hybridized to 43K arrays; total RNA from the mitotic shake-off experiment was first amplified using a modified eberwine protocol, aRNA converted to cDNA by reverse transcription with random hexamer and hybridized to 43K arrays.